EasyPfu DNA Polymerase
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Cat.No.
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Package
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10×pfu Buffer (with Mg2+)
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PC0301
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250U
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0.5ml
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PC0302
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500U
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1ml
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PC0303
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3000U
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1ml×6
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Conc. 5 U/μl Store at -20°C
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Description
EasyPfu DNA Polymerase is purified from E. coli. expressing a cloned Pyrococcus furiosus DNA polymerase gene. This polymerase possesses a proofreading 3' to 5'. exonuclease activity that provides higher fidelity than Taq. EasyPfu DNA Polymerase makes it ideal for demanding PCR applications such as site-directed mutagenesis and PCR expression cloning.
Unit Definition
One unit of EasyPfu DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-insoluble material in 30 min at 74°C.
EasyPfu DNA Polymerase Storage Buffer
50 mM Tris-HCl (pH 8.0), 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, stabilizers, and 50% (v/v) glycerol.
Quality Control
The absence of endodeoxyribonucleases and exodeoxyribonucleases confirmed by appropriate quality tests. Functionally tested in PCR.
10X EasyPfu Buffer with 20mM MgSO4
200mM Tris-HCl (pH 8.8 at 25°C), 100mM (NH4)2SO4, 100mM KCl, 1% (v/v) Triton X-100, 1mg/ml BSA and 20mM MgSO4.
Reaction Mixture Set Up
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Component
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Volume
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Final Concentration
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Template DNA
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<0.5 μg
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as required
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Forward Primer (10 μM)
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1 μl
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0.2–0.4 μM each
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Reverse Primer (10 μM)
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1 μl
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0.2–0.4 μM each
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10×Buffer
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5 μl
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1×
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2.5 mM dNTPs
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4 μl
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0.2 mM
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EasyPfu DNA polymerase
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0.5 μl
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2.5 unit
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ddH2O to final volume
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50 μl
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Not applicable
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Recommended thermal cycling conditions
94℃ 2-5 min
94℃ 30 sec
50-60℃ 30 sec 30-35 cycles
72℃ 2 min/1 kb
72℃ 5-10 min
Notes:
l MgSO4 is included in the 10×PCR Buffer at a final concentration of 2 mM, which is sufficient for most targets. For some targets, more Mg2+ may be required; use 100 mM MgSO4 prepare a titration from 2 mM to 4 mM (final concentration) in 0.25-mM increments.
we are Biotechnology manufacture with advanced equipment, consist of many biological technology specialized person, enzyme engineering technology is our core .we specialized in various kinds of dNTP (mix), dATP, dCTP, dGTP dTTP,ATP,CTP,GTP,UTP and so on for many years .All items r meet high technique standards at comparative price .we have many fixed abroad customers. Except dNTP and NTP we also produce DNA Polymerase (Taq、pfu、 M-MLV) and DNA molecular markers ,and other molecular Biology reagent. All the products passed the QC inspection strictly. We supply OEM and Bulk service for all products and keep secret strictly, hope we can establish steady and long-term business cooperation between us.
Any interested pls feel free to contact with us
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