Pfu master Mix
Pfu master Mix is a lyophilized mixture containing Pfu DNA Polymerase which is isolated from Pyrococcus furious, dNTPs, MgCl2 and PCR reaction Buffer in one PCR tube. Pfu master Mix provides robust PCR performance in a wide range of PCR applications without time-consuming optimization. Especially, this enzyme has a high PCR fidelity with improved yield and specificity. To prepare the final PCR, only primers and template DNA are added. Pfu master Mix contributes to highly reproducible PCR by reducing the risk of pipetting errors and miscalculation. Pfu mater Mix can be stored at 2-8°C allowing even faster PCR setup by eliminating thawing time.
- Premixed ready-to-use reagents containing Pfu DNA Polymerase, dNTPs, MgCl2
and reaction buffer in one PCR tube
- 3'->5' proofreading activity
- 5'->3' exonuclease activity
- Extra A addition (terminal transferase) activity
- Nuclease contamination : NO
- RNase contamination : NO
- Concentration : 1unit/20ul
- Lowest error rate
- Savings in preparation time
- Reduced risk of handling errors and contamination
- Easy handling and shipping with stability at 4°C
- Applications
- High Fidelity PCR experiments
- Blunt End Cloning
- Sequencing
- Site-Directed Mutagenesis
PCR reaction system PCR cycle
(Take a 50 μl PCR reaction system as an example)
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Template DNA
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<0.5 μg
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as required
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Forward Primer(10 μM)
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1-2 μl
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0.2-0.4 μM each
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Reverse Primer (10 μM)
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1-2 μl
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0.2-0.4 μM each
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2×Pfu PCR master Mix
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25 μl
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1×
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ddH2O to final volume
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50 μl
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Not applicable
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we are Biotechnology manufacture with advanced equipment, consist of many biological technology specialized person, enzyme engineering technology is our core .we specialized in various kinds of dNTP (mix), dATP, dCTP, dGTP dTTP,ATP,CTP,GTP,UTP and so on for many years .All items r meet high technique standards at comparative price .we have many fixed abroad customers. Except dNTP and NTP we also produce DNA Polymerase (Taqãpfuã M-MLV) and DNA molecular markers ,and other molecular Biology reagent. All the products passed the QC inspection strictly. We supply OEM and Bulk service for all products and keep secret strictly, hope we can establish steady and long-term business cooperation between us.
Any interested pls feel free to contact with us
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