Description:

EasyTaq DNA polymerase is isolated from the E. coli cloning Thermus aquaticus.The molecular weight of the product is approximately 94 kDa. EasyTaq DNA polymerase has the 5′to 3′DNA polymerase activity and 5′to 3′exonuclease activity without 3′-5′exonuclease activity.The extending speed is 1-2 kb/min.There is an “A” on  3′end.The PCR product can be cloned in TA vector.

Characteristics:

（1）      high-sensitivity（2）high amplification efficiency

Unit Definition:

One unit of Platinum Taq DNA Polymerase High Fidelity incorporates 10 nmol of deoxyribonucleotide into acid-preceptible material in 30 minutes at 74°C.

Quality Control:

This product has passed the following quality control assays: functional absence of double- and single-stranded endonuclease activity; >90% homogeneous by SDS gel electrophoresis.Each lot of EasyTaq DNA Polymerase is assayed for amplification from as little as 10 ng of human genomic DNA .

Storage Buffer

 20 mM Tris-HCl (pH 8.0)，0.1 mM EDTA，1 mM DTT，100 mM KCl，Stabilizers，50% glycerol。10×EasyTaq Buffer (+ Mg2⁺) [200 mM Tris-HCl (pH 8.4)，200 mM KCl，100 mM(NH4)2SO4 ，20 mM MgSO4，and others].

There are two types of 10×EasyTaq Buffer :with Mg²⁺ and without Mg²⁺.

The 10×Buffer without Mg²⁺ has been put on rations by 100 mM Mg₂SO4

We offer the buffer with Mg²⁺ commonly.

Reaction Mixture Set Up

Recommended thermal cycling conditions

94℃ 2-5 min

94℃ 30 sec

50-60℃ 30 sec        30-35 cycles

72℃ 1-2 kb/min

72℃ 5-10 min

we are Biotechnology manufacture with advanced equipment, consist of many biological technology specialized person, enzyme engineering technology is our core .we specialized in various kinds of dNTP (mix), dATP, dCTP, dGTP dTTP,ATP,CTP,GTP,UTP and so on for many years .All items r meet high technique standards at comparative price .we have many fixed abroad customers. Except dNTP and NTP we also produce DNA Polymerase (Taq、pfu、 M-MLV) and DNA molecular markers ,and other molecular Biology reagent. All the products passed the QC inspection strictly. We supply OEM and Bulk service for all products and keep secret strictly, hope we can establish steady and long-term business cooperation between us.

 Any interested pls feel free to contact with us